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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Plasma N-Cleaved Galectin-9 Is a Surrogate Marker for Determining the Severity of COVID-19 and Monitoring the Therapeutic Effects of Tocilizumab
doi: 10.3390/ijms24043591
Figure Lengend Snippet: Schema showing the detection systems in two types of Gal-9 ELISA. Full-length Gal-9 (FL-Gal9), and NCRD and CCRD with attached truncated linker peptides that differ in length depending on the type of proteases (N-cleaved-Gal9 and C-cleaved-Gal9, respectively) are present in human blood. The concentration of FL-Gal9 was measured using FL-Gal9 ELISA. The concentration of Tr-Gal9, a mixture of FL-Gal9 and N-cleaved-Gal9, was measured using Tr-Gal9 ELISA. The capture and detection antibodies and detected fragments in each ELISA are shown. The concentration of N-cleaved-Gal9 was calculated using both ELISA by subtracting the concentration measured by FL-Gal9 ELISA from that measured by Tr-Gal9 ELISA. Gal-9: galectin-9, ELISA: enzyme-linked immunosorbent assay, CRD: carbohydrate-recognition domain, NCRD: N-terminal CRD, CCRD: C-terminal CRD, mAb: monoclonal antibody, polyAb: polyclonal antibody.
Article Snippet: The plasma levels of Tr-Gal9 were quantified using Tr-Gal-9 ELISA, which has been previously constructed using both the capture monoclonal Ab, 9S2-3, and
Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay